Hemolysis induced by Bacillus cereus sphingomyelinase
نویسندگان
چکیده
منابع مشابه
Role of Sphingomyelinase in Infectious Diseases Caused by Bacillus cereus
Bacillus cereus (B. cereus) is a pathogen in opportunistic infections. Here we show that Bacillus cereus sphingomyelinase (Bc-SMase) is a virulence factor for septicemia. Clinical isolates produced large amounts of Bc-SMase, grew in vivo, and caused death among mice, but ATCC strains isolated from soil did not. A transformant of the ATCC strain carrying a recombinant plasmid containing the Bc-S...
متن کاملKinetic steps for the hydrolysis of sphingomyelin by Bacillus cereus sphingomyelinase in lipid monolayers.
The sphingomyelinase (Sphmase) activity degrading sphingomyelin (Sphm) monolayers shows a slow-reaction latency period before exhibiting constant rate catalysis. These two kinetic regions are regulated independently by the lateral surface pressure and by lipids that are biomodulators of cell function such as ceramide, glycosphingolipids, fatty acids, and lysophospholipids. Knowledge of the inte...
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The isolation of prumycin from cultured broths of Streptomyces kagawaensis has been reported by HATA et al.') It inhibits the growth of some bacteria and fungi,') and shows antitumor action.') The structure determined by OMURA et al.3,4) to be 4-D-alanylamino-2-amino-2,4-dideoxy-Larabinose. We isolated this antibiotic from cultured broths of a strain BMG366-UF5 which was isolated from a soil sa...
متن کاملMutation in aspartic acid residues modifies catalytic and haemolytic activities of Bacillus cereus sphingomyelinase.
Four aspartic acid residues (Asp126, Asp156, Asp233 and Asp295) of Bacillus cereus sphingomyelinase (SMase) in the conservative regions were changed to glycine by in vitro mutagenesis, and the mutant SMases [D126G (Asp126-->Gly etc.), D156G, D233G and D295G] were produced in Bacillus brevis 47, a protein-producing strain. The sphingomyelin (SM)-hydrolysing activity of D295G was completely aboli...
متن کاملPhosphatidylcholine-specific phospholipase C and sphingomyelinase activities in bacteria of the Bacillus cereus group.
Bacillus anthracis is nonhemolytic, even though it is closely related to the highly hemolytic Bacillus cereus. Hemolysis by B. cereus results largely from the action of phosphatidylcholine-specific phospholipase C (PC-PLC) and sphingomyelinase (SPH), encoded by the plc and sph genes, respectively. In B. cereus, these genes are organized in an operon regulated by the global regulator PlcR. B. an...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Biomembranes
سال: 2010
ISSN: 0005-2736
DOI: 10.1016/j.bbamem.2010.03.004